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On-farm inoculum production in temperate climates
General procedure
We have developed a method for the on-farm production of AM fungus inoculum in temperate climates that requires no input of synthetic chemicals or fumigation (Douds et al., 2005, 2006). Bahiagrass (Paspalum notatum Flugge) seedlings, colonized by AM fungi, are transplanted into black plastic bags containing a mixture of compost and vermiculite. The plants are tended over the growing season (weeded and watered as needed) and the AM fungi proliferate as roots spread throughout the compost and vermiculite mixture. Frost then kills the bahiagrass. The AM fungi naturally overwinter in the media and the inocula are ready for use the following spring. This system has successfully propagated all AM fungi tested, and produced hundreds of propagules cm-3 in a 1:4 [v/v] mixture of yard clippings compost and vermiculite (Douds et al., 2005).
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